枯草芽孢杆菌中胺氧化酶基因（yobN）的检测和相对定量：实时定量PCR的应用。
Journal of Food Science and Technology
(
IF
2.6
)
Pub Date : 2021-04-11
, DOI:
10.1007/s13197-021-05090-9
Hana Pištěková
1,
2
,
Petra Jančová
1
,
Leona Buňková
1
,
Tomáš Šopík
2,
3
,
Kristýna Maršálková
1
,
Lucie Berčíková
1
,
František Buňka
4,
5
Affiliation
Department of Environmental Protection Engineering, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001 Zlín, Czech Republic.
Centre of Polymer Systems, University Institute, Tomas Bata University in Zlín, Tř. T. Bati 5678, 760 01 Zlín, Czech Republic.
Department of Food Technology, Faculty of Technology, Tomas Bata University in Zlín, Vavrečkova 275, 76001 Zlín, Czech Republic.
Faculty of Military Leadership, University of Defence in Brno, Kounicova 65, 662 10 Brno, Czech Republic.
University Institute, Tomas Bata University in Zlín, Nad Ovčírnou 8685, 760 01 Zlín, Czech Republic.
微生物降解食品中不需要的生物胺（BA）被认为是消除其毒性的最有效方法之一。在本研究中，我们设计了两套引物用于枯草芽孢杆菌中胺氧化酶基因（yobN）和内源（管家）基因（gyrB）的检测和定量。此外，这些组可用于通过实时 PCR (qPCR) 进行 yobN 的相对定量。我们还测试了三种细菌菌株（枯草芽孢杆菌菌株：IB1a、CCM 2216、CCM 2267）在矿物培养基中为期两天的 BA 降解情况。它们的降解能力通过带有紫外检测的高效液相色谱法（HPLC/UV）进行了验证。结果显示，两种菌株显着（P < 0.05）降低组胺、酪胺、腐胺和尸胺。此外，我们的结果表明，枯草芽孢杆菌菌株的降解能力可能受到孢子形成的限制，因为编码胺氧化酶（yobN）的基因不再在孢子中表达。
"点击查看英文标题和摘要"
Detection and relative quantification of amine oxidase gene (yobN) in Bacillus subtilis: application of real-time quantitative PCR.
Degradation of undesirable biogenic amines (BAs) in foodstuffs by microorganisms is considered one of the most effective ways of eliminating their toxicity. In this study, we design two sets of primers for the detection and quantification of the amine oxidase gene (yobN) and endogenous (housekeeping) gene (gyrB) in Bacillus subtilis. Moreover, these sets can be used for relative quantification of yobN by real-time PCR (qPCR). We also tested the degradation of BAs by three bacterial strains (B. subtilis strains: IB1a, CCM 2216, CCM 2267) in a mineral medium over a two-day period. Their degradation abilities were verified by high performance liquid chromatography with UV detection (HPLC/UV). According to the results, two strains significantly (P 
