武汉三鹰生物技术有限公司

经过测试的应用Positive WB detected in   Calyculin A treated PC-3 cells, PDGF treated NIH/3T3 cells  

Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

推荐稀释比应用推荐稀释比
Western Blot (WB)   WB : 1:1000-1:5000
 
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.  
Sample-dependent, Check data in validation data gallery.  

发表文章中的应用WB    
IHC    
IF    

产品信息

28731-1-AP targets Phospho-AKT (Ser473) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用   WB, ELISA Application Description  
文献引用应用   WB, IHC, IF  
经测试反应性   human, mouse, rat  
文献引用反应性   human, mouse, rat, pig, rabbit, sheep  
免疫原   Peptide  
宿主/亚型   Rabbit / IgG  
抗体类别   Polyclonal  
产品类型   Antibody  
全称   v-akt murine thymoma viral oncogene homolog 1  
别名   AKT (Ser473), p AKT, p AKT (Ser473), p-AKT, p-AKT (Ser473)  
观测分子量   58 kDa  
GenBank蛋白编号   NM_005163  
基因名称   AKT1  
Gene ID (NCBI)   207  
RRID   AB_2881201  
偶联类型   Unconjugated  
形式   Liquid  
纯化方式   Antigen affinity purification  
UNIPROT ID   P31749  
储存缓冲液   PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.  
储存条件   Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.  

背景介绍

Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA (PubMed:20333297). This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation (PubMed:9512493). Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1 (PubMed:21464307, PubMed:8978681). AKT1 Thr-308 and Ser-473 is Phosphorylated by IKBKE and TBK1 (PubMed:15718470, PubMed:18456494, PubMed:20481595, PubMed:8978681). Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A) (PubMed:14761976). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells (PubMed:17013611). Ser-473 phosphorylation is enhanced by signaling through activated FLT3 (By similarity). Ser-473 is dephosphorylated by PHLPP (PubMed:28147277) and Ser-473 by PP2A phosphatase (PubMed:21329884).

实验方案Product Specific Protocols
WB protocol for Phospho-AKT (Ser473) antibody 28731-1-AP   Download protocol  
Standard Protocols
Click here to view our Standard Protocols